Molecular Characterization of Escherichia coli with Ciprofloxacin and Extended-Spectrum Cephalosporins Co-resistance from Patients Attending Tertiary Hospitals in Nasarawa State, Nigeria

Files

MOLECU~1.PDF (1.48 MB)

Date

2020-05-13

Authors

Nkene, I.H.
Ngwai, Y.B.
Bassey, E.B.
Abimiku, R.H.
Ibrahim, T.
Ibrahim, Y.

Journal Title

Journal ISSN

Volume Title

Publisher

Department of Microbiology, Nasarawa State University Keffi

Abstract

Antibiotic treatment of urinary tract infections caused by Escherichia coli remain the mainstay of its control. Molecular characterization of E. coli with ciprofloxacin and extended-spectrum cephalosporins co-resistance from patients attending tertiary hospitals in Nasarawa State, Nigeria was investigated. Thirty-five isolates, 13 from Dalhatu Araf Specialist Hospital Lafia (DASHL) and 22 from Federal Medical Centre, Keffi (FMCK), were obtained from previous studies. Phenotypic detection of extended spectrum beta-lactamase (ESBL) production was by double disc synergy test. Genes for ESBL and plasmid-mediated quinolones resistance (PMQR) were detected using polymerase chain reaction. Molecular diversity of the isolates was analyzed using Restriction Fragment Length Polymorphism (RFLP). Out of 35 isolates, 18 (54.5%) were ESBL producers, with higher occurrence in DASHL (54.5%) than FMCK (46.2%). The ESBL genes blaSHV, blaCTX-M and blaTEM were detected in both hospitals, with highest occurrence of 83.3% each for blaSHV and blaCTX-M in FMCK; and 66.7% for blaTEM in DASHL. The PMQR genes acc (6ʹ)-Ib-cr oqxAB, qnrB and qnrS were present in FCMK; but only qnrS was absent in DASHL. Occurrence of acc (6ʹ)-Ib-cr was highest (100.0%) in FMCK; while qnrB was highest (75.0%) in DASHL. The occurrence of strain A type isolates was higher in FMCK (100.0) than DASHL (83.33%), while no strain B type isolate in FCMK. The ESBL (blaSHV, blaCTX-M and blaTEM) and PMQR (acc (6ʹ)-Ib-cr oqxAB, qnrB and qnrS) genes were detected in the isolates. The most common circulating strain in both DASHL and FMCK was strain A, with no strain B in FCMK.

Description

Keywords

Escherichia coli, urine, ciprofloxacin, cephalosporins, extended spectrum

Citation

1. Azeez, D.A., Findik, D., Dagi, H.T. and Arslan, U. (2018). Plasmid-mediated fluoroquinolones resistance in clinical isolates Escherichia coli in Konya, Turkey.Cukurova Medical Journal, 43(2):295-300. 2. Abimiku, R.H., Ngwai, Y.B., Nkene, I.H., Tatfeng, Y. M. (2016). Molecular detection of diarrheagenicpathotypes of Escherichiacoli from diarrheic patients in Keffi, Nigeria. Microbioz Journal of Microbiology and Biomedical Research, 2(3): 1-6. 3. Bouchakour, M., Zerouali, K., Claude, J.D.P.G., Amarouch, H., El Mdaghri, N., Courvalin, P. and Timinouni, M. (2010). Plasmidmediated quinolone resistance in expanded-spectrum beta lactamase producing enterobacteriaceae in Morocco.The Journal of Infection in Developing Countries, 4(12):799–803. 4. Cattoir, V., Poirel, L., Rotimi, V., Soussy, C.J. andNordmann, P. (2007). Multiplex PCR for detection of plasmid-mediated quinolone resistance qnr genes in ESBL-producing enterobacterial isolates. Journal of Antimicrobial Chemotherapy, 60(2):394-397. 5. Chen, Y.H., Ko, W.C. and Hsueh, P.R. (2012). The role of fluoroquinolones in the management of urinary tract infections in areas with high rates of fluoroquinolone-resistant uropathogens. European Journal of Clinical Microbiology and Infectious Disease, 31:1699-1704. 6. Crémet, L., Caroff, N., Dauvergne, S., Reynaud, A., Lepelletier, D. andCorvec, S. (2011). Prevalence of plasmid-mediated quinolone resistance determinants in ESBL Enterobacteriaceae clinical isolates over a 1-year period in a French hospital. PathologieBiologie, 59(3):151–156. 7. El-bouamri, M.C., Arsalane, L., Zerouali, K., Katfy, K., El kamouni, Y. and Zouhair, S. (2015). Molecular characterization of extended spectrum beta-lactamase-producing Escherichia coli in a university hospital in Morocco, North Africa.African Journal of Urology, 21:161–166

URI

https://keffi.nsuk.edu.ng/handle/20.500.14448/5917

Collections

Articles