Molecular Characterization of Escherichia coli with Ciprofloxacin and Extended-Spectrum Cephalosporins Co-resistance from Patients Attending Tertiary Hospitals in Nasarawa State, Nigeria
| dc.contributor.author | Nkene, I.H. | |
| dc.contributor.author | Ngwai, Y.B. | |
| dc.contributor.author | Bassey, E.B. | |
| dc.contributor.author | Abimiku, R.H. | |
| dc.contributor.author | Ibrahim, T. | |
| dc.contributor.author | Ibrahim, Y. | |
| dc.date.accessioned | 2023-12-14T07:42:48Z | |
| dc.date.available | 2023-12-14T07:42:48Z | |
| dc.date.issued | 2020-05-13 | |
| dc.description.abstract | Antibiotic treatment of urinary tract infections caused by Escherichia coli remain the mainstay of its control. Molecular characterization of E. coli with ciprofloxacin and extended-spectrum cephalosporins co-resistance from patients attending tertiary hospitals in Nasarawa State, Nigeria was investigated. Thirty-five isolates, 13 from Dalhatu Araf Specialist Hospital Lafia (DASHL) and 22 from Federal Medical Centre, Keffi (FMCK), were obtained from previous studies. Phenotypic detection of extended spectrum beta-lactamase (ESBL) production was by double disc synergy test. Genes for ESBL and plasmid-mediated quinolones resistance (PMQR) were detected using polymerase chain reaction. Molecular diversity of the isolates was analyzed using Restriction Fragment Length Polymorphism (RFLP). Out of 35 isolates, 18 (54.5%) were ESBL producers, with higher occurrence in DASHL (54.5%) than FMCK (46.2%). The ESBL genes blaSHV, blaCTX-M and blaTEM were detected in both hospitals, with highest occurrence of 83.3% each for blaSHV and blaCTX-M in FMCK; and 66.7% for blaTEM in DASHL. The PMQR genes acc (6ʹ)-Ib-cr oqxAB, qnrB and qnrS were present in FCMK; but only qnrS was absent in DASHL. Occurrence of acc (6ʹ)-Ib-cr was highest (100.0%) in FMCK; while qnrB was highest (75.0%) in DASHL. The occurrence of strain A type isolates was higher in FMCK (100.0) than DASHL (83.33%), while no strain B type isolate in FCMK. The ESBL (blaSHV, blaCTX-M and blaTEM) and PMQR (acc (6ʹ)-Ib-cr oqxAB, qnrB and qnrS) genes were detected in the isolates. The most common circulating strain in both DASHL and FMCK was strain A, with no strain B in FCMK. | en_US |
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| dc.identifier.uri | https://keffi.nsuk.edu.ng/handle/20.500.14448/5917 | |
| dc.language.iso | en | en_US |
| dc.publisher | Department of Microbiology, Nasarawa State University Keffi | en_US |
| dc.subject | Escherichia coli, urine, ciprofloxacin, cephalosporins, extended spectrum | en_US |
| dc.title | Molecular Characterization of Escherichia coli with Ciprofloxacin and Extended-Spectrum Cephalosporins Co-resistance from Patients Attending Tertiary Hospitals in Nasarawa State, Nigeria | en_US |
| dc.type | Article | en_US |