Detection and molecular typing of dengue virus circulating among febrile patients in a private tertiary institution in North Central Nigeria

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Detection and typing of DENV.pdf (587.42 KB)

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2020-09-21

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DEPARTMENT OF MICROBIOLOGY, NASARAWA STATE UNIVERSITY, KEFFI.

Abstract

There is still paucity of published data on dengue virus particularly its circulating serotypes in Nigeria and Africa at large. This study was however conducted to detect and type dengue virus (DENV) serotype(s) circulating among febrile patients in a private tertiary institution in North Central Nigeria. After informed consent, 400 blood samples were collected from febrile patients at the University Health Centre. The resulting sera were screened for DENV seromarkers (IgM, IgG and NS1) using Aria Dou DENV RDT kits (CTK Biotech, Inc, San Diego, USA) while all positive samples were serotyped by PCR using type specific primers. Data obtained were analyzed using Smith’s Statistical Package (Version 2.8, California, USA) and p value of ≤0.05 was considered statistically significant. Of the 400 patients screened, 12(3.0%) were positive for acute and recent DENV infection. Age and gender of the patients were not associated with the infection (p>0.05). However, it was higher among female subjects of age ≤20 years. Of the 12 positive samples serotyped, 7(58.3%) were of DENV-3 while the remaining 5(41.7%) were not-typable. Our findings confirmed the circulation of DENV-3 in the study area. Hence, there is need for the inclusion of dengue virus test in the routine diagnosis of febrile illnesses to avoid misdiagnosis and mistreatment.

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Keywords

Dengue, Febrile illness, Infection, Nigeria, Serotypes

Citation

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URI

https://keffi.nsuk.edu.ng/handle/20.500.14448/5922

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