Genotypic characterization of hepatitis B virus among human immunodeficiency virus patients at a tertiary health care facility in North Central Nigeria

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WJARR-2020-0132.pdf (609.64 KB)

Date

2020-05-29

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Department of Microbiology, Nasarawa State University, Keffi

Abstract

Hepatitis B virus (HBV) remains a major global health problem, with approximately 257 million people chronically infected. HBV co-infection with human immunodeficiency (HIV) virus is a major public health problem especially in developing countries. In this study, the genotypes of HBV were determined among 400 consenting HIV patients accessing healthcare in Federal Medical Center, Keffi, Nigeria by Nested Multiplex PCR method. Blood samples were collected and screened for HBsAg using ACON kit (ACON Laboratories Inc, USA). HBV DNA from 18 positive samples were genotyped. Of these, 5.6% were of genotype B, 22.2% were of genotype F and 72.2% were not-typeable. This study reported the circulation of genotypes B and F in the study population with predominance of not-typable strains. To the best of our knowledge, this is the first report of genotype F in a Nigerian study.

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Keywords

Hepatitis B; HIV; Genotype; PCR; Nigeria

Citation

[1] World Health Organization. (2017). Global hepatitis programme: Global hepatitis report. [2] Oti BV, Pennap GR and Ngari HR. (2017). HBsAg and anti-HCV prevalence among pregnant women accessing antenatal care in a tertiary healthcare facility in Central Nigeria, Hepatology and Pancreas Science, 2, 110-113. [3] Stanaway JD, Flaxman AD, Naghavi M, Fitzmaurice C, Vos T, Abubakar I, AbuRaddad LJ, Assadi R, Bhala N and Cowie B. (2016). The global burden of viral hepatitis from 1990 to 2013: Findings fromthe global burden of disease Study 2013. Lancet, 388, 1081–1088. [4] Musa BM, Busell S, Borodo MM, Samaila AA and Femi OL. (2015). Prevalence of hepatitis B virus infection in Nigeria: A systematic review and meta-analysis. Nigerian Journal of Clinical Practice, 18, 163-172. [5] Tula MY and Iyoha O. (2015). A cross-sectional study on the seroprevalence of HBsAg among apparently healthy students of a tertiary institution in North-eastern Nigeria. International Journal Tropical Disease and Health, 7(3), 102-108. [6] Pennap GR, Oti BV, Alaribe AG, Ajegena SA and Galleh PR. (2017). Seroprevalence of hepatitis B and C among human immunodeficiency virus infected patients in Federal Medical Center Keffi. Journal of Advances in Microbiology, 3(4), 1-6.

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https://keffi.nsuk.edu.ng/handle/20.500.14448/5932

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