BIOSORPTION OF MANGANESE BY Bacillus subtilis AND Pseudomonas aeruginosa ISOLATED FROM WASTE DUMP SITE
dc.contributor.author | Fawehinmi, Oluseye Moronfayo | |
dc.date.accessioned | 2023-12-14T07:46:00Z | |
dc.date.available | 2023-12-14T07:46:00Z | |
dc.date.issued | 2018-05-31 | |
dc.description.abstract | In the present study, we isolated and assessed the biosorption of manganese by Bacillus subtilis and Pseudomonas aeruginosa from waste dump site. The abilities of the organisms to take up manganese were evaluated using atomic absorption spectrophotometer (AAS). The organisms were isolated using standard microbiological culture techniques. The results showed that there was higher sorption rate at temperature 37oC than at 25oC. The biosorption percentage was highest at pH 5 for both Pseudomonas aeruginosa and Bacillus subtilis with percentage biosorption of 80.4% and 75.2% respectively. The optimum pH for manganese biosorption was found to be 5. The percentage biosorption increases from pH 1to 5 and thereafter decreased from 6 to 9.Percentage biosorption for Pseudomonas aeruginosa at 37oC was 89.5% and at 25oC was 69.7%. Also, percentage biosorption for Bacillus subtilis at 37oC gave 78.4% while at 25oC, 58.3%was achieved. It was observed that Pseudomonas aeruginosa has a higher uptake than Bacillus subtilis. One-Way ANOVA test was used in this study as statistical analysis. One-Way ANOVA test for manganese biosorption by Pseudomonas aeruginosa in days has the F-value of 0.0430, P-value of 0.0507 and the treatment degree of freedom (df) of 3 while for Bacillus subtilis has the F-value of 12.1876, the P-value of 0.0024 and the treatment degree of freedom (df) of 3. The effect of pH on percentage (%) manganese biosorption by Pseudomonas aeruginosa and Bacillus subtilis has the P-value of F-value of 2.0558, 0.5005 and the treatment degree of freedom (df) of 1. The effect of temperature on percentage biosorption of manganese by Pseudomonas aeruginosa and Bacillus subtilis has the F-value of 0.3747, P-value of 1.8743 and the treatment degree (df) of 1. The use of these microorganisms with modifications using biotechnology for biosorption of heavy metals should be encouraged. | en_US |
dc.identifier.citation | A DISSERTATION SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, NASARAWA STATE UNIVERSITY KEFFI, IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF DEGREE OF MASTER OF SCIENCE IN ENVIRONMENTAL MICROBIOLOGY | en_US |
dc.identifier.uri | https://keffi.nsuk.edu.ng/handle/20.500.14448/5970 | |
dc.language.iso | en | en_US |
dc.publisher | DEPARTMENT OF MICROBIOLOGY FACULTY OF NATURAL AND APPLIED SCIENCES NASARAWA STATE UNIVERSITY, KEFFI | en_US |
dc.title | BIOSORPTION OF MANGANESE BY Bacillus subtilis AND Pseudomonas aeruginosa ISOLATED FROM WASTE DUMP SITE | en_US |
dc.type | Thesis | en_US |