Isolation, identification andScreening of Pseudomonas fluorescens from soil environment of Keffi, Nasarawa state - Nigeria for the production of Biofertilizer

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Makut et al. SP.pdf (590.31 KB)

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2018-05-05

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Department of Microbiology, Nasarawa State University Keffi

Abstract

Bio-fertilizer has been identified as an alternative to chemical fertilizer to increase soil fertility and crop production with no residual harmful effect to the environment.This study on isolation, identification andscreening of Phosphate solubilisationabilityofPseudomonas fluorescens from soil environmentwas carried out from May to September 2018. Soil samples were collected from eight different locations and Pseudomonas strainswere isolated and identified using standard microbiological methods.The 16SrRNA gene sequence analysis of the strain showed maximum similarity of 96% with Pseudomonas fluorescensreference type straindeposited in RDP GenBank. Pseudomonas strains isolated from five different sites showed clear halo zone ranging from 8-18mm. Solubilized Phosphate content produced by Pseudomonas ranged from 207.3 mg/l -273.75 mg/l.

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Bio-fertilizer, Pseudomonas Fluorescens, Phosphate Solubilisation

Citation

Ahmad F, Ahmad I, and Khan M.S (2005). “Indole acetic acid production by indigenous isolates of Azotobacter and fluorescent Pseudomonas in the presence and absence of tryptophan,” Turkish Journal of Biology, 29: 29-34. Anand S, Gowdu BJ, Kumar KSM (2007). Study of mineral phosphate solubilizing (MPS) soil bacteria and their use as bio-fertilizer. Biovistas: Future and Beyond, 3(1): 5-17. Association of Analytical Communities, (2016). Official methods of analysis of A.O.A.C International, 20th edition. A.O.A.C International, Gaithersburg, Maryland, U.SA CollavinoM.M, SansberroP.A, MroginskiL.A and Aguilar O.M(2010). ``Comparison of in vitro solubilisation activity of diverse phosphate-solubilizing bacteria native to acid soil and their ability to promote Phaseolus vulgaris growth,” BiolFertil Soils, vol. 46, pp. 727–738 De-Bashan L.E, Bashan Y. (2004). Recent advance in removing phosphorus from wastewater and its future use as bio-fertilizer. Water Res. 38:4222-4246

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https://keffi.nsuk.edu.ng/handle/20.500.14448/5907

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