ANTIBIOTIC SUSCEPTIBILITY AND PLASMID PROFILE OF SALMONELLA TYPHI FROM DOOR HANDLES IN TWO TERTIARY INSTITUTIONS IN NASARAWA STATE, NIGERIA

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dc.contributor.authorMohammed, Sidi Alhaji.
dc.date.accessioned2023-12-14T07:45:52Z
dc.date.available2023-12-14T07:45:52Z
dc.date.issued2016-11-11
dc.description.abstractContamination of door handles with antibiotic resistant pathogens is a major threat to public health and the prospect of transferring these multiple antibiotics resistance traits by enterobacteria like Salmonellae to other bacterial pathogens calls for more public health concerns. This study therefore isolated Salmonella typhi from door handles in Nasarawa State University, Keffi and Federal Polytechnic, Nasarawa and determined its antibiotics susceptibility pattern and plasmid profile. One hundred door handles from each of the two institutions, making 200 in total were sampled and 36(18.00%) Salmonella typhi were isolated. The isolates were 100% resistant to 7 out of the 10 antibiotics tested. Minimum Inhibitory Concentrations (MICs) study on selected multiple antibiotics resistant isolates showed that the isolates were susceptible to the tested antibiotics in the following order: Imipenem = Ciprofloxacin > Ceftazidime > Cefuroxime > Ampicillin > Nitrofurantoin. Fourteen (38.89%) of the multiple antibiotic resistant isolates produced beta lactamase enzymes. Conjugation experiment on the multiple antibiotics resistant S. typhi isolates showed that 7(18.42%) of the test S. typhi isolates transferred resistance plasmid gene to sensitive Proteus mirabilis and the MICs of the recipients increased significantly after conjugation. Agarose gel electrophoresis analysis of the transconjugants and the donors showed the presence of plasmid of different sizes ranging from 1600 to 2500 base pairs in both donor multiple antibiotics resistant S. typhi and transconjugants P. mirabilis. The hazardous medical implications of this resistant plasmids transfer are discussed in this report.en_US
dc.identifier.citationA DISSERTATION SUBMITTED TO THE SCHOOL OF POST GRADUATE STUDIES, NASARAWA STATE UNIVERSITY, KEFFI IN PARTIAL FULFULMENT OF THE REQUIREMENTS FOR THE AWARD OF MASTERS DEGREE IN MEDICAL MICROBIOLOGYen_US
dc.identifier.urihttps://keffi.nsuk.edu.ng/handle/20.500.14448/5955
dc.language.isoenen_US
dc.publisherDepartment of Mass Communication, Nasarawa State University Keffi.en_US
dc.titleANTIBIOTIC SUSCEPTIBILITY AND PLASMID PROFILE OF SALMONELLA TYPHI FROM DOOR HANDLES IN TWO TERTIARY INSTITUTIONS IN NASARAWA STATE, NIGERIAen_US
dc.typeThesisen_US

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