Articles

Permanent URI for this collection

Now showing 1 - 1 of 1

Open Access
EFFECT OF Allium sativum ON PHOSPHOLIPASE FROM Naja mossambica (COBRA)
(Department of Biochemistry, Nasarawa State University Keffi, 2011-11-15) A., Abdullahi.; A. B., Salau.; E. C., Egwin.; H. L., Muhammad.; F. S., Adefolalu.; K., Ebisine
Methanolic extract of Allium sativum (garlic) was investigatedfor its inhibitory action on both crude and partially purified phospholipase activity from Naja mossambica venom. The purification scheme employed both ammonium sulphate precipitation and size exclusion (gel Alteration) chromatography in sephadex G-50. The enzyme was inhibited by different concentrations of the extract in a dose dependent pattern. Double reciprocal plot of the enzyme activity against both substrate and inhibitor concentration showed an uncompetitive pattern of inhibition. The and Km of both crude and partially purified phospholipase gave 67*l05M/min, 1.28mg/ml and 98*l(fM/™in, 2.41mg/ml respectively. Inhibition constant(Ki) to determine binding affinity of inhibitor to enzyme for both crude and partially purified gave 0.17mg/ml and 0.04mg/ml respectively suggesting a higher binding affinity and may be a good sou rce ofpoten tial inhibitor of phospholipase