Thesis and Dissertations

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Browsing Thesis and Dissertations by Author "Mowobi, Gbolahan Gabriel."

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    ASSESSMENT (RAPD) OF AFRICAN MAHOGANY (Khaya senegalensis (Desr.) A. Juss) AND NEEM (Azadirachta indica A.Juss) AND THEIR HERBAL PRODUCTS MANAGEMENT OF DIABETES IN NASARAWA STATE AND THE F.C.T., ABUJA
    (Department of Plant Science and Biotechnology,Nasarawa State University Keffi., 2016-10-10) Mowobi, Gbolahan Gabriel.
    Khaya senegalensis and Azadirachta indica are common plants that have been used in the management of diabetes. In the present investigation, RAPD Marker Assessment was carried out on Herbal Products from Khaya senegalensis and Azadirachta indica being used in the Management of Diabetes in Nasarawa State and Federal Capital Territory (FCT). Eleven decamer oligonucleotide primers were screened in the RAPD analysis for authentication of claim for genuine and adulterant samples. The Deoxyribonuclic Acid (DNA) isolated from the dried herbal samples was used as templates in polymerase chain reactions with the eleven primers. Five of the eleven primers gave species specific reproducible unique amplicons. The unique amplicons obtained in PCR amplification and the data generated from the amplification of the DNA were subjected to Numerical Taxonomy System of Statistic (NTSYS). From the phylogenetic tree generated among the 24 samples based on PCR product, it was observed that the relatedness was 37.5%. The analysis also showed that there was no strong interrelationship between herbal samples and plant sample at that index. At the end of the investigation, the result showed that the herbs contained both Khaya senegalensis and Azardirachta indica as they claim though; the quantity is low (37.5%).