Citric Acid Production by Aspergillus niger and Trichoderma viride Using Hydrolysed Potato Peels Substrate
dc.contributor.author | Makut, M.D. | |
dc.contributor.author | Ekeleme, I.K. | |
dc.date.accessioned | 2023-12-14T07:42:33Z | |
dc.date.available | 2023-12-14T07:42:33Z | |
dc.date.issued | 2018-02-13 | |
dc.description.abstract | Aim: Production of citric acid by Aspergillus niger and Trichoderma viride isolated from soil in Keffi, Nigeria using Hydrolysed potato peels substrate. Place and Duration of the Study: This study was carried out in the Department of Microbiology Faculty of Natural and Applied Sciences Nassarawa State University Keffi, Nigeria, between February and July 2017. Methodology: Aspergillus niger and Trichoderma viride were isolated from soil in Keffi and identified using standard microbiology methods. Hydrolysed potato peels substrate - soy beans cake (ps) and potato peel - groundnut cake (pg) production media were prepared by following standard fermentation conditions. The citric acid produced was estimated using Gas Chromatography/Mass Spectrometry (GC/MS) method respectively. Results: At pH 5.5 after 72 hours of incubation the highest production of citric acid was produced by T. viride 176.33±20.10 mg/l on ps and A. niger 91.11±21.22 mg/l on ps. At pH 6.0 T. viride produced highest on ps 211.40±7.10 mg/l and A. niger 170.22±10.81 mg/l on ps while at pH 6.5 A. niger produced highest 181.09±20.8 mg/l on ps and T. viride 98.76±19.8 mg/l on ps with highest total Sugar consumption and dry cell mass were 16.19±20.01 g/l and 13.17±1.04 g/l while after 144 hours of incubation at pH 6.0 A. niger produced highest citric acid 404.53 ± 14.32 mg/l on ps and T. viride produced 297.60±8.31mg/l on ps highest total Sugar consumption and dry cell mass were 19.91±2.01g/l and 17.0±15.11 g/l. Conclusion: The maximum citric acid production obtained during the course of study was 404.53±14.32 mg/l on ps by A. niger and at pH 6.0. Also potato peels showed that it can be alternative carbon source for citric acid production as cheaper source of carbon. | en_US |
dc.identifier.citation | 1. Ates S, Dingil N, Bayraktar E, Mehmetoglu U. Enhancement of citric acid production by immobilized and freely suspended Aspergillus niger using silicone oil. Process Biochemistry. 2002;38:433– 436. 2. Finogenova TV, Morgunov IG, Kamzolova SV, Chernyavskaya OG. Organic acid production by the yeast Yarrowia lipolytica: A review of prospects. Applied Biochemistry and Microbiology. 2005:41: 418–425. 3. Francielo V, Patricia M, Fernanda SA. Apple pomace: A versatile substrate for biotechnological applications. Critical Reviews in Biotechnology. 2008;281–12. 4. Helen Shnada Auta, Khadijat Toyin Abidoye, Hauwa Tahir, Aliyu Dabai Ibrahim, and Sesan Abiodun Aransiola Citric acid production by Aspergillus niger cultivated on parkia biglobosa fruit pulp. International Scholarly Research Notices. 2014;21(20):8-19. 5. Makut MD, Ade-Ibijola OB. Citric acid producing fungi found in the soil environment of Keffi Metropolis, Nassarawa State. International Resources Journal of microboiolgy. 2012;3(7):240- 245. 6. Carlos RS, Vandenberghe LPS, Rodrigues C, Pandey A. New perspectives for citric acid production and application. Food Technology and Biotechnology. 2006;44(2):141–149. 7. Varsha G Shetty. Production and optimization of citric acid by Aspergillus niger Using molasses and corncob. International Journal of Pharmaceutical Science. 2015;7(5):152-157 | en_US |
dc.identifier.uri | https://keffi.nsuk.edu.ng/handle/20.500.14448/5906 | |
dc.language.iso | en | en_US |
dc.publisher | Department of Microbiology, Nasarawa State University Keffi | en_US |
dc.subject | Aspergillus niger; Trichoderma viride; ps and pg substrate. | en_US |
dc.title | Citric Acid Production by Aspergillus niger and Trichoderma viride Using Hydrolysed Potato Peels Substrate | en_US |
dc.type | Article | en_US |