Evaluation of Polymorphisms at Heat Shock Protein 90 Gene by High Resolution Melting Assays for Potential Heat Tolerance among Nigerian Zebu Cattle Breeds

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dc.contributor.authorOnasanya, Gbolabo O
dc.contributor.authorMsalya, G.M
dc.contributor.authorThiruvenkadan, A.K
dc.contributor.authorSreekumar, C.
dc.contributor.authorTirumurugaan, G.K
dc.contributor.authorSanni, M.
dc.contributor.authorDecampos, J.S
dc.contributor.authorAmusan, A. S
dc.contributor.authorFafiolu, A.O
dc.contributor.authorOlowofeso, Oladeji
dc.contributor.authorOkpeku, M.
dc.contributor.authorYakubu, Abdulmojeed
dc.contributor.authorIkeobi, C.O.N
dc.date.accessioned2023-12-11T13:47:14Z
dc.date.available2023-12-11T13:47:14Z
dc.date.issued2020-03-14
dc.description.abstractHeat Shock Protein (HSP) 90 gene is a member of HSPs subfamily that act as molecular chaperons whenever animals come under thermal stress. The genes fulfill essential roles of providing cellular protection, immune response, protein synthesis, protein folding and unfolding, protection from cellular stress, inhibitory apoptosis and adaptation. This study was designed to analyze polymorphisms of HSP 90 and to evaluate their influence on heat tolerance among selected Nigerian zebu. The polymorphisms were also used to determine genetic relationship among the animals. About 450 bp of bovine HSP 90 including part of coding region in exon 3 was sequenced in 90 DNA samples representing four Nigerian zebu namely White Fulani (WF), Sokoto Gudali (SG), Red Bororo (RB) and Ambala (AM). Sequencing was done using an automated ABI-DNA Sequencer. Editing was accomplished using chromatogram analyses on SeqMan Ngen Tool. Rooted phylogenetic tree was constructed using MEGA 5.2 software. In total, 11 genetic variants were determined. Five of these (PRP, RED, ORG, LMN and YLO) were major variants detected in over 70% of the samples. Six (6) were classified as minor variants detected in two breeds or less and in 29.1% of the samples. The GRN and NBL were only detected in RB and SG breeds respectively. We found a shared homology and common ancestral lineage among the breeds. Furthermore, the genetic structure of Nigerian zebu has a common clade architecture to those of goats, sheep, yak, buffalo, camel, horse and other taurines. The gene is conserved among wide range of animals and as such it can serve as one of bio-markers for selection and breeding programmes for thermotolerance in wide range of livestock animals under thermal stress. The variant groups could be further interrogated for possible specific effects on thermotolerance performance of zebu in hot tropical environments.en_US
dc.identifier.citationYakubu, A et al. (2020), Evaluation of Polymorphisms at Heat Shock Protein 90 Gene by High Resolution Melting Assays for Potential Heat Tolerance among Nigerian Zebu Cattle Breeds.en_US
dc.identifier.urihttps://keffi.nsuk.edu.ng/handle/20.500.14448/2869
dc.language.isoenen_US
dc.publisherDepartment of Animal Science, Nasarawa State University, Keffi.en_US
dc.subjectBio-marker, Bos indicus, Genetic Variants, Phylogeny, Thermal Assaulten_US
dc.titleEvaluation of Polymorphisms at Heat Shock Protein 90 Gene by High Resolution Melting Assays for Potential Heat Tolerance among Nigerian Zebu Cattle Breedsen_US
dc.typeArticleen_US

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