Browsing by Author "Tarfa, F.D."

Now showing 1 - 2 of 2
  • Thumbnail Image
    ItemOpen Access
    EVALUATION OF HYPOGLYCAEMIC AND ANTIOXIDANT ACTIVITIES OF CALOTROPIS PROCERA LEAF IN ALLOXAN- INDUCED DIABETIC RATS
    (Department of Biochemistry and Molecular Biology, Nasarawa State University Keffi, 2018-10-10) Ajiboso, S.O.; Tarfa, F.D.
    The hypoglycemic and antioxidant activities of aqueous extract of Calotropis procera leaf in alloxan-induced diabetic rats were evaluated in this study. Adult Wistar rats of mean weight 110.0±1.4g were randomised into six groups (A-F), group A (non-diabetic) received orally 0.5ml of distilled water once daily for 10 days, groups B, C, D, E and F were made diabetic with alloxan (150 mg/kg body weight i.p). Groups C, D, E and F also received once daily 0.5ml of metformin (2.5 mg/kg b.w p.o), 25, 50 and 100 mg/kg body weight o.p. of the extract respectively. Standard procedures were used to determine blood glucose, body weight, hepatic ascorbic acid, reduced glutathione and activities of catalase, glutathione peroxidase and superoxide dismutase. The results obtained revealed significant elevation (p<0.05) of blood glucose, liver MDA and reduction (p<0.05) of hepatic ascorbic acid content, reduced glutathione level, body weight and activities of catalase, glutathione peroxidase and superoxide dismutase in distilled water- treated diabetic rats. The increased and reduced parameters were reverted back to the range of the non-diabetic control animals in the groups treated with metformin and extract particularly 100mg/kg b.w. dosage.
  • Thumbnail Image
    ItemOpen Access
    RESPONSE OF SOME HEPATIC CARBOHYDRATES METABOLIZING ENZYMES TO AQUEOUS EXTRACT OF CALOTROPIS PROCERA LEAF IN DIABETIC RAT
    (Department of Biochemistry and Molecular Biology, Nasarawa State University Keffi, 2019-01-01) Ajiboso, S.O.; Tarfa, F.D.
    Diabetes mellitus is a global disease affecting mankind regardless of age, race, gender, economic status and geographical location. Diabetes mellitus is associated with biochemical complications such as alteration and fluctuation in enzyme activity. The response of some carbohydrates metabolizing enzymes to aqueous extract of Calotropis procera leaf in alloxanised rat was studied under standard conditions. In the present study, there was significant increase (p>0.05) in blood glucose level (21.80 – 22.70mmol/L) and activities of hexokinase (5.70unit/g tissue) and phosphofructokinase (17.00unit/g tissue) while activities of glucose-6-phosphatase (56.80unit/g tissue) and fructose-1,6-biphosphatase (6.60unit/g tissue) were significantly decreased (p>0.05) in alloxanised groups when compared to non-diabetic group. Administration of aqueous extract of Calotropis procera leaf lowered the high blood glucose level and reverted back the values of the enzymes back to normal in extract administered groups. The extract performance in terms of hypoglycaemic activity and reversion of enzyme activity back to normal was dose – dependent, with highest performance exhibited by 100 mg/kg dose of extract, this dosage (100 mg/kg) compared favourably in performance with metformin – a standard antidiabetic drug. From the present study, the 100 mg/kg dose of the extract can be used to correct diabetic complications and biochemical disorders associated with these enzymes.