Browsing by Author "Talabi, Adewale O."

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    Molecular Diagnosis of Subclinical African Trypanosoma vivax Infection and Association with Physiological Indices and Serum Metabolites in Extensively Managed Goats in the Tropics
    (Department of Animal Science, Nasarawa State University, Keffi., 2013-06-27) Sanni, Timothy M; Onasanya, Gbolabo O; Adefenwa, Mufliat A; Yakubu, Abdulmojeed; Ikeobi, C.O.N; Adebambo, O.A; Talabi, Adewale O.; Ozoje, M.O; Wheto, M.; Takeet, Michael I.; Peters, S.O; Donato, Marcos De; Thomas, Bolaji N.; Imumorin, I.G
    Trypanosomosis remains a major challenge to livestock production in much of tropical Sub-Saharan Africa, while diagnosis and treatment still depend on inefficient parasitological techniques. Endemic infections depend on animal reservoirs with subclinical parasitemia. We report molecular diagnosis of subclinical Trypanosoma vivax (T. vivax) infection using polymerase chain reaction (PCR) for the first time in Nigerian goats and associate parasite presence with gross physiological traits and serum metabolites in extensively managed Nigerian goats. PCR was used to amplify a 400 bp DNA fragment of the parasite genome in 205 goats across three geographical zones of the country. Results showed a high subclinical infection rate (SCIR) of 71.7% in the total goats examined. Overall SCIRs of 71%, 75.9% and 55.6% were recorded in West African Dwarf, Red Sokoto and Sahel goats respectively, while geographical SCIRs were 71.2% (Southwest), 75% (Northwest) and 70% (Northeast). T. vivax presence had significant (P < 0.05) effect on respiratory rate and is associated with higher creatinine levels in sera. Logistic regression analyses with Hosmer-Lemeshow goodness- of-fit showed that respiratory rate is the most important predictive trait for the presence of T. vivax infection (P < 0.05). Goats appear to be a viable reservoir for T. vivax infection of other livestock. Molecular diagnosis of subclinical trypanosomosis using PCR could be useful for large scale epidemiological studies, early diagnosis of subclinical infection and treatment of the disease in extensively managed tropical goats.
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    Molecular-based detection of sub-clinical African Trypanosoma vivax infection and its association with some selected serum biochemical references and blood electrolytes in four traditionally bred Nigerian native Sheep.
    (Department of Animal Science, Nasarawa State University, Keffi., 2018-09-12) Yakubu, Abdulmojeed; Onasanya, Gbolabo O; Sanni, Timothy M; Amusan, A. S; Decampos, J.S; Talabi, Adewale O.; Ozoje, M.O; Balogun, Joshua Babalola; Wheto, M.; Ikeobi, C.O.N
    Trypanosomosis remains a major challenge to livestock production in much of tropical Sub- Saharan Africa, while diagnosis and treatment still depends on inefficient parasitological techniques. Endemic infections of trypanosomosis depend on animal reservoirs with sub-clinical parasitemia. We report molecular diagnosis of sub-clinical Trypanosoma vivax (T. vivax) infection using polymerase chain reaction (PCR) for the first time in Nigerian sheep and associate parasite presence with gross physiological traits and biochemical references in extensively managed tropical sheep. PCR was used to amplify a 400 bp DNA fragment of the parasite genome in 161 sheep of both sexes across four geographical zones of Nigeria. Results showed a high sub-clinical infection rate (SCIR) of 73.9% in the total sheep investigated. Overall, SCIRs of 85.4%, 75%, 62.5% and 72.5% were recorded in Balami, West African Dwarf, Uda and Yankassa sheep, respectively; while geographical SCIRs were 73.5 % (South-West), 71.7 % (North-West), 73.5 % (North-East) and 88.0 % (North-Central). SCIRs of 73.5 % were found in ewes and 76.3 % in rams. T. vivax infection presence had a significant (p<0.05) effect on blood urea nitrogen (BUN), Alanine transaminase or Alanine aminotransferase (AST) and alkaline phosphatase (ALP) where infected sheep had a higher ALP levels of 242.24 +21.72 IU/dl than non-infected sheep (189.86 +10.77 IU/dl). Also T. vivax infected sheep had higher AST (185.92+13.90 IU/L) than non-infected counterparts (167.31+15.58 IU/L). Tropical sheep appear to be a fertile reservoir for T. vivax infection of other livestock. Molecular diagnosis of sub-clinical trypanosomosis using PCR-based assay is suitable for large scale epidemiological studies of trypanosomosis, early diagnosis of sub-clinical infection and treatment of the disease in extensively managed tropical sheep