Browsing by Author "Suchi, Kyeswet Nanret"

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    Detection and molecular typing of dengue virus circulating among febrile patients in a private tertiary institution in North Central Nigeria
    (DEPARTMENT OF MICROBIOLOGY, NASARAWA STATE UNIVERSITY, KEFFI., 2020-09-21) Haruna, Isa Mohammed; Suchi, Kyeswet Nanret; Pennap, Grace Rinmecit
    There is still paucity of published data on dengue virus particularly its circulating serotypes in Nigeria and Africa at large. This study was however conducted to detect and type dengue virus (DENV) serotype(s) circulating among febrile patients in a private tertiary institution in North Central Nigeria. After informed consent, 400 blood samples were collected from febrile patients at the University Health Centre. The resulting sera were screened for DENV seromarkers (IgM, IgG and NS1) using Aria Dou DENV RDT kits (CTK Biotech, Inc, San Diego, USA) while all positive samples were serotyped by PCR using type specific primers. Data obtained were analyzed using Smith’s Statistical Package (Version 2.8, California, USA) and p value of ≤0.05 was considered statistically significant. Of the 400 patients screened, 12(3.0%) were positive for acute and recent DENV infection. Age and gender of the patients were not associated with the infection (p>0.05). However, it was higher among female subjects of age ≤20 years. Of the 12 positive samples serotyped, 7(58.3%) were of DENV-3 while the remaining 5(41.7%) were not-typable. Our findings confirmed the circulation of DENV-3 in the study area. Hence, there is need for the inclusion of dengue virus test in the routine diagnosis of febrile illnesses to avoid misdiagnosis and mistreatment.
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    Parallel and Concurrent Infection of Dengue Virus and Plasmodium falciparum among Patients with Febrile Illnesses Attending Bingham University Health Centre, Karu, Nigeria
    (DEPARTMENT OF MICROBIOLOGY, NASARAWA STATE UNIVERSITY, KEFFI., 2020-09-09) Suchi, Kyeswet Nanret; Haruna, Isa Mohammed; Ademola, Olutunde Adegbite; Pennap, Grace Rinmecit
    Aims: This study was conducted to determine the parallel and concurrent infection of dengue virus and Plasmodium falciparum among patients with febrile illnesses attending Bingham University Health Centre, Karu, Nigeria. Study Design: The study was a cross sectional study. Place and Duration of Study: Department of Medical Microbiology and Parasitology, Jos University Teaching Hospital, Jos, Department of Microbiology, Nasarawa State University, Keffi and 68 Nigerian Army Reference Hospital, Yaba-Lagos, between February and July 2017. Methodology: Blood samples were collected from 400 patients with febrile illnesses at the University Health Centre. The resulting sera was screened for dengue virus seromarkers (IgM, IgG and NS1) using Aria Dou dengue virus RDT kits (CTK Biotech, Inc, San Diego, USA) while malaria parasitemia was detected by Giemsa stained thick and thin film microscopy. Data collected were analysed using Smith’s Statistical Package (version 2.8, California, USA) and P value of ≤ 0.05 was considered statistically significant. Results: Of the 400 patients screened, 12(3.0%) were positive for dengue virus, 20(5.0%) for malaria parasite while 10(2.5%) for dengue/malaria co-infection. Infection with dengue virus and malaria parasite was found to be higher among female subjects aged ≤30 years. However, age and gender were not significantly associated with both infections in this study (P > 0.05). Conclusion: Our findings confirmed the presence of dengue virus infection in the study area which probably may have been misdiagnosed and mistreated. Hence, differential diagnosis of febrile illnesses should not only be limited to malaria and typhoid as is always the case in our health care centres.