Browsing by Author "Sreekumar, Chirukandoth"

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    Evolutionary study of HSP 90AA1gene among Nigerian zeb breeds of cattle revealed shared ancestry
    (Department of Animal Science, Nasarawa State University, Keffi., 2019-10-22) Yakubu, Abdulmojeed; Onasanya, Gbolabo O; Thiruvenkadan, Aranganoor K; Sreekumar, Chirukandoth; Tirumurugaan, Gopalan K.; Msalya, George M.; Muyideen, Sanni T.; Okpeku, M.; Ikeobi, C.O.N; Fafiolu, Adeboye O.; Olowofeso, Olajide
    Heat shock protein (HSP) 90AA1 gene is a member of HSPs sub-family that act as molecular chaperons whenever animals come under the assaults of thermal stress, they fulfill essential roles of providing cellular protection, immune response, inhibitory apoptosis and adaptation to thermal assault. A total of ninety (90) adult bulls from Nigerian Zebu cattle breeds comprising of White Fulani (25), Sokoto Gudali (21), Red Bororo (21) and Ambala (23) sampled from northern parts of Nigeria. We reported that rooted evolutionary study based on Neighbour-joining dendrogram of HSP 90AA1 sequences of White Fulani, Ambala, Sokoto Gudali and Red Bororo Nigerian Zebu breeds of cattle revealed that HSP 90AA1 sequences of four Nigerian cattle breed showed shared homology which is suggestive of common ancestral lineage. Similarly, nucleotide sequences of HSP 90AA1 gene in four Nigerian B. Indicus and those of goat, sheep, yak, buffalo, camel, horse and taurine were also constructed. Our results showed that sequences of Nigerian cattle breeds and those of goat, sheep, yak, buffalo, camel, horse and taurine at HSP 90AA1 gene locus suggested that these animals had shared ancestry and common evolution. Therefore, the extent of relatedness detected among Nigerian breeds of cattle and those of selected mammalian species indicated that HSP 90AA1 gene is conserved among wide range of animals and as such it can be used as potential bio-marker for thermo-tolerance in wide range of livestock animals under assaults of thermal stress. Keywords: HSP 90AA1 gene, thermal stress, phylogenetic analyses, cattle
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    Heterozygous Single-Nucleotide Polymorphism Genotypes at Heat Shock Protein 70 Gene Potentially Influence Thermo-Tolerance Among Four Zebu Breeds of Nigeria
    (Department of Animal Science, Nasarawa State University, Keffi., 2021-12-15) Yakubu, Abdulmojeed; Onasanya, Gbolabo O; Msalya, George M.; Thiruvenkadan, A.K; Sreekumar, Chirukandoth; Tirumurugaan, Gopalan K.; Fafiolu, A.O; Adeleke, M.A; Ikeobi, C.O.N; Okpeku, M.
    Genetic variants at heat shock protein 70 gene and their influence on heat stress (HS) tolerance were studied among selected Nigeria zebu, namely, 25 White Fulani (WF), 21 Sokoto Gudali (SG), 21 Red Bororo (RB), and 23 Ambala (AM). Detection of single nucleotide polymorphism (SNP) followed by determination of genotype and genotypic frequency was made among the selected breeds. The heat tolerance coefficient (HTC) was determined from thermo-related parameters including body temperature, rectal temperature, and respiratory rate. Thermo-Tolerance was evaluated through the SNP– thermo-parameter relationship. Statistical analyses were done using the GLM procedure in SAS. A quantitative real-time/high-resolution melting-based assay detected twelve genetic variants. Five of these were common and shared across all breeds of cattle. Of the remaining seven variants, three were specifically identified in AM, two in SG, and two in RB. Also, SNPs were evaluated and four unique SNPs (C151T, C146T, G90A, and C219A) were identified. Heterozygous animals had lower HTC suggesting their potential to withstand HS than homozygous counterparts. The WF and RB animals had significantly lower values for all parameters (BT, RT, RR, and HTC) compared to AM and SG breeds. Thermo-related parameters were significantly different (P < 0.001), and it is recommended that screening of SNPs in zebu is needed to enable selection for improved thermo-tolerance.
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    Single nucleotide polymorphisms at heat shock protein 90 gene and their association with thermo-tolerance potential in selected indigenous Nigerian cattle
    (Department of Animal Science, Nasarawa State University, Keffi., 2020-06-28) Yakubu, Abdulmojeed; Onasanya, Gbolabo O; Msalya, G.M; Thiruvenkadan, A.K; Sreekumar, Chirukandoth; Tirumurugaan, Gopalan K.; Okpeku, M.; Sanni, Timothy M; Decampos, J.S; Amusan, S.A; Olowofeso, Oladeji; Fafiolu, A.O; Ikeobi, C.O.N
    Heat shock protein (HSP) 90 gene provides protection and adaptation to thermal assault and certain polymorphisms have been associated to heat tolerance in humans and animals. Single nucleotide polymorphisms (SNPs) of HSP 90 gene were used to evaluate the scientific basis of heat tolerance in four zebu breeds of Nigeria. The DNA was extracted from skin tissue of 90 adult bulls representing White Fulani (WF), Sokoto Gudali (SG), Red Bororo (RB), and Ambala (AM). The SNPs were determined in DNAs using PCR, sequencing, and visualization and bio-editing by chromatogram in SeqMan Ngen tool. Subsequently, respective genotypes were constructed and genotypic and allelic frequencies were computed. Also, body parameters related to heat stress (HS) including body temperature (BT), rectal temperature (RT), and respiratory rates (RR) were taken for each animal before biological sampling and heat tolerance coefficient (HTC) was calculated. We detected four SNPs distinct/specific for each breed as follows: change from thymine (T) to guanine (G) at position 116 (T116G) in RB, G to cytosine (C) at 220 (G220C) in SG, G to adenine (A) at two positions, 346 (G346A) and 390 (G390A) in AM and WF, respectively. Heterozygous SNPs showed significantly lower values (P < 0.0001) for BT, RT, RR, and HTC than homozygous genotypes at all positions.We hypothesize that animals with heterozygous SNPs in exon 3 of HSP 90 may be tolerant to HS. These SNPs can be used as bio-markers for screening large populations of cattle for tolerance to hot tropical conditions in Nigeria and other sub-humid places.