Browsing by Author "Ngwai, Y. B."

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    ANTIBIOGRAM OF NON-SORBITOL FERMENTING ESCHERICHIA COLI ISOLATED FROM ENVIRONMENTAL SOURCES IN KEFFI, NIGERIA
    (Deepartment of Microbiology, Nasarawa State University, Keffi., 2014-01-01) Ngwai, Y. B.; Gyar, S. D.; Pennap, Grace Rinmecit; Makut, M.D.; Ishaleku, D.; Corosi, S. M.; Nkene, I. H.; Uzoamaka, N.
    Non-sorbitol fermenting Escherichia coli, particularly 0157:H7, are major public health concern. This study was undertaken to isolate and identify non-sorbitol fermenting (NSF) E. coli from stool samples and food sources in Keffi metropolis. Four hundred and thirty (430) samples of vegetables (100), raw beef (110), undercooked beef (60), local fresh milk product ("Nono”: 50), cow dung (10) and human stool (100) were collected from five different locations in Keffi metropolis and NSF E. coli was isolated and confirmed by culture on Sorbitol MacConkey agar, Eosine Methylene Blue agar and biochemical tests namely, Indole, Methyl Red, Voges-Poskauer and citrate test (IMViC). Antibiotics susceptibility tests using standard procedure were carried on the isolates. E. coli was detected in 298 (69.3%) samples. Of the positive samples, 64 (21.5%) were NSF E coli with high detection in raw beef (43.8%) and cow dung (40.0%); and 234 (78.5%) were sorbitol-fermenting (SF) strains with high detection in all sources. The NSF E. coli were highly susceptible to septrin (79.7%), peflacine (68.8%), streptomycin (71.9%), gentamicin (62.5%) and ofloxacin (70.3%); but less to ampicillin (12.5%), augmentin (32.8%), ceporex (32.8%), nalidixic acid (43.8%) and ciprofloxacin (48.4%). There were 46 different resistance phenotypes with AU, CPX, SXT, S, PN, CEP, OFX, NA, PEF; AU, CPX, PN, CEP, NA; AU, PN, CEP, NA, CN; PN, CEP, NA, CN; and CPX, PN, NA dominating at 4.7 % each. MAR was observed in all the isolates with resistance to 4 and 5 antibiotics being more frequent at 23.4% and 21.9% respectively. The lowest MAR index was 0.3. In conclusion, non-sorbitol fermenting E. coli was shown to contaminate some cabbage vegetables, raw beef, undercooked beef, and "Nono” consumed in Keffi metropolis. The NSF E. coli were more susceptible to septrin, aminoglycosides and ofloxacin; and originate from an environment where antimicrobials are freely available and misused. __ _
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    Antibiotic Resistance Profile of Escherichia coli from Urine of Patients with Suspected Urinary Tract Infections in Federal Medical Centre, Keffi, Nigeria
    (Department of Microbiology, Nassarawa State University Keffi,, 2019-08-22) Nkene, I. H.; Ngwai, Y. B.; Bassey, E.B.; Pennap, Grace Rinmecit; Makut, M.D.
    Aims: This study investigated the antibiotic resistance profile of Escherichia coli from the urine of patients with suspected urinary tract infections in Federal Medical Centre, Keffi, Nigeria. Study Design: Cross-sectional study. Place and Duration of Study: Sample was obtained from the Federal Medical Center, Keffi and analyzed at Nasarawa State University, Keffi, Nigeria, between January and April 2018. Methodology: Three hundred and eighty urine samples were collected and E. coli was isolated and identified using standard microbiological methods. Antimicrobial Susceptibility Testing for the isolates was carried out and interpreted as described by the Clinical and Laboratory Standards Institute. Results: The occurrence of the bacterium was 12.9% (49/380). The occurrence in relation to the gender of the patients was higher in the female (15.5%) than the male (9.8%); in relation to age, it was highest at 11-20 years (23.5%) but lowest at > 50 years (2.3%). The isolates were more resistant to ampicillin (81.6%), streptomycin and sulphamethoxazole/ trimethoprime (75.0%) but less resistant to gentamycin (30.6%), and imipenem (22.4%). The occurrences of different classes of resistance were multidrug resistance (MDR) (93.9%) and pan drug resistance (4.2%). Most of the isolates were more resistant to the commonly prescribed antibiotic and were also MDR isolates. Conclusion: The need to review antibiotic use by the hospital is thus justified.