Browsing by Author "Murtala, Zaharadeen Ibrahim"

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    Evaluation of the efficacy and optimization of indigenous microbial isolate for herbicides (Paraquat Dichloride, Glyphosate, and Glyphosate isopropylamine) degradation
    (Department of Microbiology, Nassarawa State University Keffi,, 2021-08-20) Murtala, Zaharadeen Ibrahim; Makut, M.D.; Abdullahi, Ari Omale; Abubakar, Magaji Umar
    The presence of herbicides in soil is a serious problem for the environment. Studies on degradation of Herbicide (Paraquate dichloride (PD), Rake out (RO) and Gobara (GB)) by bacteria and fungi species isolated from soil environment in Keffi Metropolis Nigeria were carried out. A total of twenty (20) soil samples were collected. The bacteria and fungi were isolated from the soil and identified using standard microbiological methods. The herbicides utilization was determined using Atomic Adsorption Spectrometer. The effect of temperature on utilization of the herbicides by Enterobacter asburiae at 26OC ranges from 1.23±0.11 mg/ml for PD, 1.14±0.29 mg/ml for RO and 0.53±0.86mg/ml for GB, Pseudomonas aeruginosa utilization ranges from 1.45±0.17 mg/ml for PD, 1.17±0.35 mg/ml, for RO 1.12± 0.82mg/ml for GB. Aspergillus flavus ranges from 2.12±0.19 mg/ml for PD, 2.00±0.03 mg/ml for RO and 2.02±0.57 mg/ml for GB, Fusarium redolens were 2.19±0.26 mg/ml for PD, 2.15±0.08 mg/ml for RO and 1.92±0.16 mg/ml for GB. Effect of incubation time on microbial herbicides degradations: for E. asburiae on PD it ranges from day 1 with 0.24±0.37 mg/ml to day 20 with 2.06±0.11 mg/ml. for P. aeruginosa on PD ranges from day 1 with 0.38±0.08 mg/ml to day 20 with 2.39±1.45 mg/ml. The Utilization of herbicides by A. flavus on PD ranges from day 1 with 0.10±0.01 mg/ml to day 20 with 2.29±0.12 mg/ml. for F. redolens in PD, it ranges from day 1 with 0.27±0.08 mg/ml to day 20 with 2.57±0.27mg/ml. The process of degradation of herbicide has become very attractive as it allows for removal of herbicide over a relatively broad range of pH and temperature.
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    Molecular and Phylogenic Identifications of Potential Herbicide degrading Micro organisms from Contaminated Farmland in Keffi, Nasarawa State, Nigeria
    (Department of Microbiology, Nassarawa State University Keffi,, 2021-01-28) Makut, M.D.; Murtala, Zaharadeen Ibrahim
    Over the past years, the continuous use of herbicides has raised increasing concern mainly due to their massive pollution of the environment. To address this problem, the herbicide-degrading microorganism might be very promising. Method: In the present study, a total of twenty (20) soil samples were collected, bacteria and fungi were isolated from the soil and identified using standard microbiological and molecular studies. Results: The results revealed that a total of 2 bacterial (Enterobacter asburiae and Pseudomonas aeruginosa) and 2 fungi (Aspergillus flavus and Fusarium redolens) strains were isolated from the soil samples collected from herbicide contaminated soil in Keffi, Nasarawa State, Nigeria. The E. asburiae and P. aeruginosa had 75.0%, and 100% occurrence while the fungi isolates including the A. flavus and F. redolens had 75.0%, and 100% occurrence respectively. The 16S rDNA and ITS1F analysis confirmed the identity of the bacteria and fungi genus. Phylogenetic analysis suggested the bacteria strains were closely related to Pseudomonas aeruginosa strain CIFRI DTSB1 and Enterobacter sp revealed a close relatedness with Enterobacter asburiae RD-DAROS-04 strain, whereas, the fungi strain revealed a closely relatedness of Fusarium redolens strainTIST190421511. Conclusion: In conclusion, the microbial strains including Pseudomonas aeruginosa strain CIFRI DTSB1, Entrobacter asburiae RD-DAROS-04, and Fusarium redolens strainTIST190421511 were the major microbial habitant of pesticide-contaminated farmland in Keffi, and thus represent the herbicide degrading microorganism in this region. Further studies on the herbicide degrading properties of these microbial strains are underway.