Browsing by Author "Manogaran, Motharasan"

Now showing 1 - 2 of 2
  • Thumbnail Image
    ItemOpen Access
    Isolation and Growth Characterization of an Acrylamide-degrading Bacillus sp. strain UPM2021n Isolated from the Juru River
    (Department of Biochemistry and Molecular Biology, Nasarawa State University Keffi, 2022-12-12) Muhammad, Abdullahi Ubana; Adamu, Faggo Abdullahi; Manogaran, Motharasan; Yasid, Nur Adeela; Shukor, Mohd Yunus
    Polyacrylamide, in which acrylamide is the primary component, degrades back into acrylamide over time. Major amounts of acrylamide can be found in soil. The bioremediation strategy of using microbes to break down acrylamide is gaining popularity in many parts of the world. Several acrylamide-degrading bacteria have been isolated from sediment from the Juru River’s bank. The best isolate was a bacterium identified tentatively as Bacillus sp. strain UPM2021n based on cultural, colony morphology and biochemical tests. According to early studies, ideal growth parameters included a pH range of 6.5 and 7.0 and a temperature range of 25 to 35 degrees Celsius. Both glucose and sucrose performed at a similar level in supporting the growth of this bacterium on acrylamide as the sole nitrogen source. The highest growth occurs in between 300 and 500 mg/L of acrylamide, resulting in a growth of nearly 7.7 log CFU/mL with a nett growth of about 4 Log CFU/mL as compared to the control. Growth was nearly tolerated at the highest concentrations tested, which was 1500 mg/L and growth completely ceased at higher concentrations Toxic heavy metals tested such as mercury, copper, chromium, and cadmium showed that mercury strongly hampered growth on acrylamide whilst other metal ions such as copper, lead, cadmium, and chromium showed from 30 to 60% inhibition. The relatively high tolerance of acrylamide makes this bacterium suitable for remediation of soil contaminated with acrylamide whilst its sensitivity to heavy metals chiefly mercury means metal-chelating or sequestering compounds must be added to soil contaminated with both acrylamide and heavy metals.
  • Thumbnail Image
    ItemOpen Access
    Isolation and Growth Characterization of an Acrylamide-degrading E. cloacae strain UPM2021a Isolated from a Paddy Field
    (Department of Biochemistry and Molecular Biology, Nasarawa State University Keffi, 2022-12-24) Muhammad, Abdullahi Ubana; Adamu, Faggo Abdullahi; Manogaran, Motharasan; Yasid, Nur Adeela; Shukor, Mohd Yunus
    Acrylamide is a suspected carcinogen and a global pollutant. The presence of acrylamide in the soil is a major source of this chemical. Microbe-mediated acrylamide breakdown as a bioremediation technique is gaining popularity across the world. Several bacteria capable of digesting acrylamide have been identified in paddy field soils. The best isolate was a bacterium identified tentatively as E. cloacae strain UPM2021a based on cultural, colony morphology and biochemical tests. According to early studies, ideal growth parameters included a pH range of 6.5 and 7.5 and a temperature range of 25 to 35 degrees Celsius. Acrylamide dosages of up to 2500 mg/L were explored as a single nitrogen supply. The greatest growth occurs between 300 and 1000 mg/L of acrylamide, resulting in an approximate nett growth of 3 log CFU/mL when compared to the control. Growth was practically tolerated at 1700 mg/L, and growth stopped entirely at concentrations above 2000 mg/L. Toxic heavy metals such as mercury, copper, chromium, and cadmium hampered acrylamide development with mercury being the strongest inhibitor whilst other metal ions such as copper, cadmium, and chromium show from 30 to 50% inhibition whilst lead was the least inhibiting. The relatively high tolerant of acrylamide makes this bacterium suitable for remediation of soil contaminated with acrylamide whilst its sensitivity to heavy metals chiefly mercury means metal-chelating or sequestering compounds must be added to soil contaminated with both acrylamide and heavy metals.