Browsing by Author "Jodi, S.M."

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    Antibacterial and Antibiofilm Properties of the Crude Ethanolic, Methanolic and Aqueous Bark and Seed Extracts of Citrullus lanatus Fruit
    (Department Of Microbiology, Faculty Of Natural And Applied Sciences, Nasarawa State University, Keffi., 2018-05-06) Francis, O.I.; Jodi, S.M.; Nkene, I.H.; Abimiku, R.H.; Ngwai, Y.B.; Ibrahim, T.
    Medicinal plants represent rich sources of potential antimicrobial agents. Studies on antibacterial and biofilm inhibitory properties of the ethanolic, methanolic and aqueous bark and seed extracts of Citrullus lanatus (C. lanatus) fruit was carried out. The bark and seed of C. lanatus were obtained from Karu market, Abuja and extracted separately with ethanol, methanol and water by cold maceration method. The antibacterial activities of the extracts were evaluated using agar well diffusion method against clinical isolates of Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Salmonella typhi. The Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of the extracts were determined using agar dilution method.
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    Antimicrobial Resistance Profile and Extended Spectrum Beta-Lactamase Resistance Genes in Escherichia coli from Poultry Droppings in Nasarawa, Nigeria
    (Department Of Microbiology, Faculty Of Natural And Applied Sciences, Nasarawa State University, Keffi., 2021-05-21) Tama, S.C.; Ngwai, Y.B.; Pennap, G.R.I.; Nkene, I.H.; Abimiku, R.H.; Jodi, S.M.
    Aims: This study investigated the antimicrobial resistance profile and extended spectrum betalactamase resistance genes of Escherichia coli isolated from droppings of from selected poultry farms in Nasarawa, Nigeria. Study Design: Investigative Place and Duration of Study: Department of Microbiology, Nasarawa State University, Keffi, between November 2019 and February 2020. Methodology: A total of 90 samples from poultry droppings were collected from selected farms. Escherichia coli was isolated from the samples using standard cultural and microbiological methods. Antibiotic susceptibility testing and minimum inhibitory concentrations were evaluated as described by the Clinical and Laboratory Standards Institute (CLSI). The detection of extended-spectrum betalactamase (ESBL) production in E. coli isolates was carried out using double disc synergy test. In addition, molecular detection of ESBL genes was carried out using Polymerase Chain Reaction (PCR) method. Results: The prevalence of E. coli was 100%. Antibiotic resistances of E. coli were recorded as follows: streptomycin (S: 94.4%), sulphamethoxazole / trimethoprim (SXT: 90.0%), ampicillin (AMP: 88.9%), gentamicin (CN: 68.9%), amoxicillin/clavulanic acid (AMC: 55.6%), ciprofloxacin (CIP: 41.1%), cefoxitin (FOX: 35.6%), ceftazidime (CAZ: 34.4%), cefotaxime (CTX: 22.2%), and imipenems (IPM: 17.8%). The most common antibiotic resistant resistance phenotype was AMPCTX-CAZ-CIP-CN (11.1%). Multiple antibiotic resistance (MAR) was observed in 97.7% (88/90) of the isolates, with the common MAR index being 0.5 (33.3%). Twenty five of the thirty beta-lactam resistant isolates (83.3%) were confirmed ESBL producers. The 25 ESBL positive isolates carried bla genes as follows: blaTEM (11/25, 44.0%) and blaCTX-M (18/25, 72.0%). blaSHV was not found in any isolate. Conclusion: E. coli isolated from the droppings of selected poultry farms in Nasarawa were less resistant to imipenem, cefotaxime, ceftazidime and cefoxitin in the study location. This implies that the antibiotics are useful in the treatment of infection caused by E. coli. Also, ESBL-positive E. coli isolates harbored ESBL genes, with blaCTX-M as the most common.
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    Antimicrobial resistance profile and molecular detection of MecA gene in methicillin resistant Staphylococcus aureus from patients in selected general hospitals in Abuja municipal, Nigeria
    (Department Of Microbiology, Faculty Of Natural And Applied Sciences, Nasarawa State University, Keffi., 2019-05-08) Odogwu, D.A.; Parom, S.K.; Jodi, S.M.; Bassey, E.B.; Abimiku, R.H.; Nkene, I.H.; Ngwai, Y.B.
    Staphylococcus aureus (S. aureus) is globally recognized as an important pathogen associated with both hospital and community acquired infections. Studies on antibiotic resistance profile of S. aureus and carriage of mecA gene in methicillin resistant isolates from patients attending selected general hospitals in Abuja Municipal, Nigeria was carried out. Three hundred and sixty (360) clinical samples (200 urine, 50 high vaginal swabs, 60 ear swab and 40 wound swabs) were collected from Asokoro General Hospital (AGH), Garki Hospital Abuja (GHA) and Wuse General Hospital (WGH); and S. aureus was isolated and identified using standard microbiological methods. Antibiotic susceptibility testing of the isolates was carried out using disc diffusion method. Molecular detection of mecA gene in methicillin resistant isolates was carried out using the polymerase chain reaction method. The total occurrence of S. aureuswas 15.3% (55/360); and the occurrence in relation to the selected hospitals was high in GHA (22.7%) and low in AGH (10.3%). The occurrence of S. aureus was highest in wound swabs in all the hospitals in the order: GHA (47.1%) > AGH (40%) > WGH (35.7%). The isolates from all the hospitals were highly (≥ 50.0%) resistant to all the antibiotics tested; but moderately (≤ 40.0%) to gentamicin and levofloxacin. The occurrence of multi-drug resistant (MDR) isolates in the selected hospitals was high in GHA (27%) but low in AGH (12%). Of the 32 oxacillin resistant isolates, mecA gene was detected in 30 (93.8%). The S. aureus isolates were less resistant to gentamicin and levofloxacin and most of the oxacillin resistant isolates harbored mecA gene.
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    Antimicrobial Resistance Profile of Escherichia coli Isolated from Urine of Patients in Nagari Allah Magani Hospital, Keffi, Nigeria
    (Department Of Microbiology, Faculty Of Natural And Applied Sciences, Nasarawa State University, Keffi., 2018-05-05) Eko, K.O.; Jodi, S.M.; Nkene, I.H.; Abimiku, R.H.; Ibrahim, T.; Ngwai, Y.B.
    Aim:This study investigated antimicrobial resistance in E. coli recovered from urine of patients attending Nagari Allah Magani Hospital, Keffi, Nigeria. Place and Duration of Study: Department of Microbiology, Nasarawa State University, P.M.B. 1022, Keffi, Nasarawa State, Nigeria; between January 2018 and July 2018. Methodology: Escherichia coli was isolated and identified from the urine of suspected UTIs patients by culture, microscopy and biochemical tests. Sample: We included 248 patients; (116 men, 132 women;age range 5-65 years). Antimicrobial Susceptibility Testing of the isolates was carried out in accordance with the Clinical and Laboratory Standards Institute (CLSI) method.
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    Antimicrobial Resistance Profile of Escherichia coli Isolated from Urine of Patients in Selected General Hospitals in Abuja Municipal, Nigeria
    (Department Of Microbiology, Faculty Of Natural And Applied Sciences, Nasarawa State University, Keffi., 2018-05-02) Eghieye, M.O.; Jodi, S.M.; Bassey, B.E.; Nkene, I.H.; Abimiku, R.H.; Ngwai, Y.B.
    This study investigated the antimicrobial resistance profile of Escherichia coli from urine of patients with suspected urinary tract infections (UTIs) in selected general hospitals in Abuja Municipal, Nigeria. Four Hundred and Thirty urine samples were collected between September 2017 and May 2018 from patients attending Asokoro General Hospital (AGH), Garki Hospital Abuja (GHA) and Wuse General Hospital (WGH); and E. coli was isolated and identified by culture, microscopy and biochemical tests. The overall occurrence of E. coli was 52 (12.1%). The occurrences in relation to the hospitals were of the order: GHA (14.7%) > WGH (12.6%) > AGH (9.0%). The highest (50%) occurrence was at age 41-50 years in WGH, and the lowest (4.3%) was at age 31-40 years in AGH.