Browsing by Author "Ishaya, I.O."
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Item Open Access Isolation, Identification and Screening of Pseudomonas Fluorescens from Soil Environment of Keffi, Nasarawa State - Nigeria for the Production of Bio- Fertilizer(Department of Microbiology, Nassarawa State University Keffi,, 2017-05-11) Makut, M.D.; Ishaya, I.O.; Ombugadu, A.; Onawola, O.O.; Abikoye, E.T; Mohammed, S.A.Bio-fertilizer has been identified as an alternative to chemical fertilizer to increase soil fertility and crop production with no residual harmful effect to the environment.This study on isolation, identification andscreening of Phosphate solubilisationabilityofPseudomonas fluorescens from soil environmentwas carried out from May to September 2018. Soil samples were collected from eight different locations and Pseudomonas strainswere isolated and identified using standard microbiological methods.The 16SrRNA gene sequence analysis of the strain showed maximum similarity of 96% with Pseudomonas fluorescensreference type straindeposited in RDP GenBank. Pseudomonas strains isolated from five different sites showed clear halo zone ranging from 8-18mm. Solubilized Phosphate content produced by Pseudomonas ranged from 207.3 mg/l -273.75 mg/l.