Browsing by Author "Ibrahim, K."

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    Adherence Of Escherichia Coli To Urinary Catheter a function Of time, temperature and cell physiology
    (Department Of Microbiology, Faculty Of Natural And Applied Sciences, Nasarawa State University, Keffi., 2005-05-02) Ngwai, Y.B.; Ibrahim, K.; Ijele, I.G.
    The in-vitro adhesion of Escherichia coli to urinary catheter was investigated by standard procedure Adhesion depended on time and temperature lower adhesion densities were observed when the contact time was only 2h or 6h.
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    Detection of anti-Chlamydia trachomatis antibodies in Patients with Acquired Immune Deficiency Syndrome in Abuja, Nigeria
    (Department Of Microbiology, Faculty Of Natural And Applied Sciences, Nasarawa State University, Keffi., 2008-05-14) Izebe, K.S.; Ngwai, Y.B.; Ezeunala, M.; Ajoku, G.A; Oladosu, P.; Yusuf, Y.; Ibrahim, K.; Oladepo, D.; Inyang, U.S.
    Chlamydia trachomatis (CT) infections are among the sexually transmitted diseases known to increase the risk for human immunodeficiency virus infection. Serum samples from 34 consenting AIDS patients which attended the Government-approved Antiretroviral Treatment (ART) Facility at the National Institute for Pharmaceutical Research and Development (NIPRD), Abuja between April 2005 and March 2006 were screened by enzyme immunoassay (EIA) for the presence of anti-CT antibodies using ImmunoComb® Chlamydia Bivalent IgG Test kit (Orgenics, Israel). Anti-CT antibodies were detected in ten (29.4%) of the thirty-four patients tested. The detection rate was higher among the females (33.3%) than the males (23.1%). Patients of the age group 31-45 years had the highest detection of anti-C. trachomatis antibodies, followed by those of age group 16-30 years. The result of the present study suggests the presence of anti-CT antibodies in AIDS patients, and reinforces the need for routine screening for anti-CT antibodies as a necessary intervention to reduce the burden of chlamydial diseases and to reduce the risk of HIV and its spread in Nigeria. The outcome of this study also provides justification for the possible inclusion of anti-chlamydial agents in the National AIDS Management Plan to treat associated C. trachomatis infections.
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    In vitro adherence of antibiotic-resistant Escherichia coli to biomaterial surfaces: Effect of conditioning film
    (Department Of Microbiology, Faculty Of Natural And Applied Sciences, Nasarawa State University, Keffi., 2006-05-07) Ngwai, Y.B.; Onaolapo, J.A.; Ehinimidu, J.O.; Ibrahim, K.
    In our recent study of patients attending a government hospital and a private medical laboratory in the Nigerian city of Zaria. we not only reported an increase in antibiotic resistance in urinary isolates of Escherichia coli but also the fact that the resistant isolates originated from an environment in which antibiotics were frequently used. The implication of the resistance on the adherence characteristics of these isolates is the main objective of this study. Using a laboratory model, we assessed the in vitro adherence to biologically inert surfaces of five representative antibiotic-resistant isolates with a view to finding any possible relationship between their resistance and adherence. Bacteria adhered poorly to the surfaces Adhesion was enhanced, reduced or uninfluenced by conditioning the adhering surface with urine or serum depending on the adhering bacteria or type of biomaterials. There was no apparent relationship between the antibiotic resistance of these isolates and their adherence Our observations suggest that the risk of biomatenal-associated infections is determined by both the adhering bacteria and type of biomaterial.
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    SCREENING FOR ANTIMICROBIAL POTENTIAL OF THE CRUDE METH ANOLIC EXTRACT OF THE LEAVES OF PHYSALIS ANGULATA L. AND ITS FRACTIONS
    (Department Of Microbiology, Faculty Of Natural And Applied Sciences, Nasarawa State University, Keffi., 2006-05-11) Ajoku, G.A.; Ibrahim, K.; Ijele, I.; Ezeunala, M.; Abdulrahim, M.E.; Izebe, K.S.; Oladosu, P.; Ngwai, Y.B.; Mohammed, S.B.; Ya'aba, Y.; Oladepo, D.; Inyang, U.S.
    The methanolic crude extract of die leaves of Physalis angulata was screened for its antimicrobial poten. against Escherichia coli. Pseudomonas aeroginosa. Staphylococcus aureus. Candida albicans, Bacillus spp and Proteus spp using agar dilution method. Surface streaking was employed as the method of inoculation of die organisms on the agar. The crude extract showed significant activity against Bacillus spp and Proteus spp at 2.5mg/ml. On separation of the crude extract, ten out of sixteen fractions of the crude extract exhibited significant activity against Candida albicans and Proteus spp with MIC between 0:2 5 m g/ml - 0.5mg/mL Seven fractions had MIC between 0.25mg/ml- 0.5mg/ml. against Escherichia coli, while five fractions had MIC between 0.5ml - LOmg/mi against Bacillus spp. Five fractions showed activity against Pseudomonas aeroginosa and Staphylococcus aureus at MIC between l.Omg/ml - 2.0mg/mI. Phytochcmical screening of the crude extract revealed the presence of tannins, phlabotannins, phenols. Steroids, saponins and terpenes. It is interesting that some fractions of the methanolic crude extract showed activity against Pseudomonas aeroginosa among other microorganisms, This point to the fact that this study may lead to the development of a new antimicrobial to add to the few existing to combat difficult infections due to Pseudomonas aeroginosa which is resistant to most commonly used antimicrobials.