Browsing by Author "Gyar, S. D."

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    ANTIBIOGRAM OF NON-SORBITOL FERMENTING ESCHERICHIA COLI ISOLATED FROM ENVIRONMENTAL SOURCES IN KEFFI, NIGERIA
    (Deepartment of Microbiology, Nasarawa State University, Keffi., 2014-01-01) Ngwai, Y. B.; Gyar, S. D.; Pennap, Grace Rinmecit; Makut, M.D.; Ishaleku, D.; Corosi, S. M.; Nkene, I. H.; Uzoamaka, N.
    Non-sorbitol fermenting Escherichia coli, particularly 0157:H7, are major public health concern. This study was undertaken to isolate and identify non-sorbitol fermenting (NSF) E. coli from stool samples and food sources in Keffi metropolis. Four hundred and thirty (430) samples of vegetables (100), raw beef (110), undercooked beef (60), local fresh milk product ("Nono”: 50), cow dung (10) and human stool (100) were collected from five different locations in Keffi metropolis and NSF E. coli was isolated and confirmed by culture on Sorbitol MacConkey agar, Eosine Methylene Blue agar and biochemical tests namely, Indole, Methyl Red, Voges-Poskauer and citrate test (IMViC). Antibiotics susceptibility tests using standard procedure were carried on the isolates. E. coli was detected in 298 (69.3%) samples. Of the positive samples, 64 (21.5%) were NSF E coli with high detection in raw beef (43.8%) and cow dung (40.0%); and 234 (78.5%) were sorbitol-fermenting (SF) strains with high detection in all sources. The NSF E. coli were highly susceptible to septrin (79.7%), peflacine (68.8%), streptomycin (71.9%), gentamicin (62.5%) and ofloxacin (70.3%); but less to ampicillin (12.5%), augmentin (32.8%), ceporex (32.8%), nalidixic acid (43.8%) and ciprofloxacin (48.4%). There were 46 different resistance phenotypes with AU, CPX, SXT, S, PN, CEP, OFX, NA, PEF; AU, CPX, PN, CEP, NA; AU, PN, CEP, NA, CN; PN, CEP, NA, CN; and CPX, PN, NA dominating at 4.7 % each. MAR was observed in all the isolates with resistance to 4 and 5 antibiotics being more frequent at 23.4% and 21.9% respectively. The lowest MAR index was 0.3. In conclusion, non-sorbitol fermenting E. coli was shown to contaminate some cabbage vegetables, raw beef, undercooked beef, and "Nono” consumed in Keffi metropolis. The NSF E. coli were more susceptible to septrin, aminoglycosides and ofloxacin; and originate from an environment where antimicrobials are freely available and misused. __ _