Browsing by Author "Ezeunala, M."

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    Detection of anti-Chlamydia trachomatis antibodies in Patients with Acquired Immune Deficiency Syndrome in Abuja, Nigeria
    (Department Of Microbiology, Faculty Of Natural And Applied Sciences, Nasarawa State University, Keffi., 2008-05-14) Izebe, K.S.; Ngwai, Y.B.; Ezeunala, M.; Ajoku, G.A; Oladosu, P.; Yusuf, Y.; Ibrahim, K.; Oladepo, D.; Inyang, U.S.
    Chlamydia trachomatis (CT) infections are among the sexually transmitted diseases known to increase the risk for human immunodeficiency virus infection. Serum samples from 34 consenting AIDS patients which attended the Government-approved Antiretroviral Treatment (ART) Facility at the National Institute for Pharmaceutical Research and Development (NIPRD), Abuja between April 2005 and March 2006 were screened by enzyme immunoassay (EIA) for the presence of anti-CT antibodies using ImmunoComb® Chlamydia Bivalent IgG Test kit (Orgenics, Israel). Anti-CT antibodies were detected in ten (29.4%) of the thirty-four patients tested. The detection rate was higher among the females (33.3%) than the males (23.1%). Patients of the age group 31-45 years had the highest detection of anti-C. trachomatis antibodies, followed by those of age group 16-30 years. The result of the present study suggests the presence of anti-CT antibodies in AIDS patients, and reinforces the need for routine screening for anti-CT antibodies as a necessary intervention to reduce the burden of chlamydial diseases and to reduce the risk of HIV and its spread in Nigeria. The outcome of this study also provides justification for the possible inclusion of anti-chlamydial agents in the National AIDS Management Plan to treat associated C. trachomatis infections.
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    SCREENING FOR ANTIMICROBIAL POTENTIAL OF THE CRUDE METH ANOLIC EXTRACT OF THE LEAVES OF PHYSALIS ANGULATA L. AND ITS FRACTIONS
    (Department Of Microbiology, Faculty Of Natural And Applied Sciences, Nasarawa State University, Keffi., 2006-05-11) Ajoku, G.A.; Ibrahim, K.; Ijele, I.; Ezeunala, M.; Abdulrahim, M.E.; Izebe, K.S.; Oladosu, P.; Ngwai, Y.B.; Mohammed, S.B.; Ya'aba, Y.; Oladepo, D.; Inyang, U.S.
    The methanolic crude extract of die leaves of Physalis angulata was screened for its antimicrobial poten. against Escherichia coli. Pseudomonas aeroginosa. Staphylococcus aureus. Candida albicans, Bacillus spp and Proteus spp using agar dilution method. Surface streaking was employed as the method of inoculation of die organisms on the agar. The crude extract showed significant activity against Bacillus spp and Proteus spp at 2.5mg/ml. On separation of the crude extract, ten out of sixteen fractions of the crude extract exhibited significant activity against Candida albicans and Proteus spp with MIC between 0:2 5 m g/ml - 0.5mg/mL Seven fractions had MIC between 0.25mg/ml- 0.5mg/ml. against Escherichia coli, while five fractions had MIC between 0.5ml - LOmg/mi against Bacillus spp. Five fractions showed activity against Pseudomonas aeroginosa and Staphylococcus aureus at MIC between l.Omg/ml - 2.0mg/mI. Phytochcmical screening of the crude extract revealed the presence of tannins, phlabotannins, phenols. Steroids, saponins and terpenes. It is interesting that some fractions of the methanolic crude extract showed activity against Pseudomonas aeroginosa among other microorganisms, This point to the fact that this study may lead to the development of a new antimicrobial to add to the few existing to combat difficult infections due to Pseudomonas aeroginosa which is resistant to most commonly used antimicrobials.