Browsing by Author "Bassey, A."

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    Antimicrobial Resistance profile of Escherichia coli from Urine of Patients in Nigerian National Petroleum Corporation Medical Services, Abuja, Nigeria
    (Department of Microbiology, Nasarawa State University Keffi, 2020-05-13) Bassey, A.; Istifanus, Haruna Nkene; Bassey, B.E.; Abimiku, Rejoice Helma; Parom, S.K.; Ngwai, Y.B.
    Escherichia coli (E. coli) is among the most predominant organisms causing urinary tract infections (UTIs) in humans. Studies on antimicrobial resistance in E. coli from urine of patients in Nigerian National Petroleum Corporation Medical Services, Abuja, Nigeria was carried out. Exactly 362 urine samples of patients with suspected UTIs were collected and E. coli was isolated and identified using standard microbiological methods. The antimicrobial susceptibility testing of the isolates was carried out and interpreted in accordance with Clinical and Laboratory Standards Institute protocol. Of the 362 urine samples, the occurrence of E. coli was 45(12.4%); highest age-related occurrence at 21-30yrs of 18(20.5%); and higher in female (15.6%) than male (7.6%). The 45 isolates had highest resistance to ampicillin 43(95.6%) and lowest resistance to gentamicin 13(28.9%). All the 45 isolates were multiple antibiotic resistant (MAR) isolates with MAR indices of > 0.2, and the commonest indices being 0.6 and 0.7 with 10(22.2%) and 8(17.8%) occurrences respectively. The order of occurrence of categories of antibiotic resistance in the 45 isolates was: multi-drug resistance (66.7%) > extensive drug resistance (17.8%) > non-multidrug resistance (13.3%) > pan drug resistance (2.2%). A further study on molecular diversity of the antimicrobial resistant E. coli from urine in the study location is ongoing. 1926
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    Phenotypic and Molecular Detection of Extended Spectrum β-Lactamase in Escherichia coli from Patients in Nigerian National Petroleum Corporation Medical Services, Abuja, Nigeria
    (Department Of Microbiology, Faculty Of Natural And Applied Sciences, Nasarawa State University, Keffi., 2018-05-06) Bassey, A.; Ngwai, Y.B.; Bassey, B.E.; Nkene, I.H.; Abimiku, R.H.; Parom, S.K.
    Aim: The study was conducted to determine the presence of extended spectrum beta-lactamase (ESBL) among Escherichia coli isolates recovered from the urine of patients in Nigerian National Petroleum Corporation Medical Services, Abuja, Nigeria using a phenotypic and molecular method. Methodology: Forty five (45) confirmed E. coli recovered from urine of patients with suspected UTIs were obtained; Phenotypic detection of ESBL was done on isolates resistant to cefotaxime and ceftazidime by the double disc synergy test method. Molecular detection of ESBL genes in phenotypically confirmed ESBL producers was done using Polymerase Chain Reaction. Results: Of the 40 cefotaxime and ceftazidime-resistant isolates tested, 12 (30.0%) were phenotypically confirmed as ESBL producers. ESBL genes were detected in the order (blaTEM: blaSHV: blaCTX-M): 75.0%: 58.3%: 25.0%. Some (16.7%) of the ESBL isolates harboured both blaTEM and blaSHV; and some harboured other combinations of the ESBL namely blaTEM/blaCTX-M (8.3%), blaSHV/blaCTX-M (8.3%) and blaTEM/blaSHV/blaCTX-M (8.3%). Conclusion: Many of the E. coli isolates were ESBL producers; and they harboured blaTEM genes most frequently. Further studies on molecular diversity of the ESBL producing E. coli from urine in the study location should be carried out.