Department of Microbiology

Permanent URI for this community

https://keffi.nsuk.edu.ng/handle/20.500.14448/3874

Browse

Browsing Department of Microbiology by Author "Abdel-Wahab, G F. H."

Now showing 1 - 1 of 1
  • Thumbnail Image
    ItemOpen Access
    REGULATION OF THE AFLATOXIN PRODUCTION OF ASPERGILLUS FLAVUS ISOLATE BY SOME PLANT ESSENTIAL OILS
    (FACULTY OF NATURAL AND APPLIED SCIENCE NASARAWA STATE UNIVERSITY, KEFFI., 2000-08-03) Hafez, E. E.; El-Sohaimy, S.; Zytoin, M.; El-Saadani, M.; Abdel-Wahab, G F. H.; Basu, S. K.
    Four concentration ( 0,01, 0,05, o.1and 0.5%V/V) °f five plant essential oils from different platinum pimpinella anisum amara (DC.) Buchheim and Lactura" Rlcmus cmmun's L„ Prunus dulcis (Mill.) D.A. Webb v<= broth against A flavu™ Sate for L Wfref,tested '^OmL of sterilized Sabouraud-Yeast extral period, the four AFs (B B G and cm u '°n °f af atoxin (AF) production. At the end of the incubatiion samples were quantitatively measured ShSSS by chloroform' Spitted on TLC and the reference exhibited total inhibition of the four tvnes nf ap L°'Jh?- essential 0lls of p- anisum and M. chamomile 0.01 % concentration with little effen/nn f Production in all concentrations except M. chamomilla s • (DDRT-PCR) were SSutSSmiStSH9r0Wth- Molecular Studies based the differential disPlathe chitinase gene as arbitrary primer We used^S8^ and the uP're9ulated bands (9enes) us,n£ system of the fungus against the PrioceQ„f ,, 6 chltinase gene as a genetic marker of the defense* and a single down-regulated band appearing with the used essential plant oils. we detected three putative up regulated bands and a single down regulated band appearing with the control.